Throughput

Performance parameters*

HiSeq

Read length Run time (days) # of Reads per lane Filtered data/passing Q.C. (MB/lane)
1 x 75 bp 3 170 million 12750
1 x 100 bp 4 170 million 17000
2 x 75 bp 7 340 million 25500
2 x 100 bp 9 340 million 34000
* assumes optimal cluster densities which cannot be guaranteed.

GB = gigabase, 10^9; MB = 10^6

Please use the guide here to determine how many lanes of sequencing are needed for your sample.