Advances in single cell RNA quantification techniques have enabled comprehensive study of cell subpopulations within a heterogeneous population. The GemCode™ Technology from 10X Genomics, which combines microfluidics with molecular barcoding and custom bioinformatics software enable 3’ mRNA counting from thousands of single cells.
The Chromium™ Single Cell 3’ Solution
Single cells, reagents and a single Gel Bead containing barcoded oligonucleotides are encapsulated into nanoliter-sized GEMs (Gel Bead in emulsion) using the GemCode Technology. Lysis and barcoded reverse transcription of polyadenylated mRNA from single cells are performed inside each GEM. High-quality next generation sequencing libraries are finished in single bulk reaction. Finally, the Chromium™ Software Suite is utilized for processing, analysis and visualization of single cell gene expression data.
Key advantages of the Chromium Single cell RNA-Seq
- High-throughput single cell transcriptomic measurements enable discovery of gene expression dynamics for profiling individual cell types
- Complete practical solution for single cell analysis Identify rare cell types in heterogeneous populations
- More efficient than leading academic droplet systems
- Encapsulate up to 48,000 cells in 10 minutes
- Wide dynamic range